凝胶回收与PCR产物纯化试剂盒-核酸纯化-试剂-生物在线

凝胶回收与PCR产物纯化试剂盒

商家询价

产品名称：凝胶回收与PCR产物纯化试剂盒

英文名称： NucleoSpin Extract II (250)

产品编号： 740609.250

产品价格： 0

产品产地：德国

品牌商标： MN(Macherey-Nagel)

更新时间： null

使用范围： null

广州伟杰生物科技有限公司

联系人 :
地址 : 广州市荔湾区西湾路广雅前街2号荔海商务大厦310室
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所在区域 : 广东
电话 : 189****9166 点击查看
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邮箱 : weijiebio@189.cn

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产品详情

PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• Two applications in one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)


Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 400 µL PCR reaction mixture < 400 mg TAE / TBE agarose gel
Fragment size	50 bp–approx. 20 kbp
Typical recovery	70–95%
A260/A280	1.8–1.9
Elution volume	15–30 µL
Preparation time	10 min/6 preps
Binding capacity	25 µg

Applications

• Purification of PCR products • Extraction of DNA from agarose gels
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as: - nucleotides, primers - enzymes - mineral oil - PCR additives (e.g., salts, betaine, DMSO) - detergents (e.g., Tween 20, Triton X-100) - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)
• Support protocol for DNA extraction from polyacrylamide gels • Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”) • Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis