Donkey normal serum (2 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot
Rabbit purified IgG, F(ab)'2 fragment (2 mg)
Rabbit IgG, F(ab)'2 fragment is provided in 10 mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.Purity >95 % by SDS-PAGE.